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computer aided sperm analysis system casa  (Hamilton Thorne Ltd)

 
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    Hamilton Thorne Ltd computer aided sperm analysis system casa
    Computer Aided Sperm Analysis System Casa, supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/computer aided sperm analysis system casa/product/Hamilton Thorne Ltd
    Average 86 stars, based on 1 article reviews
    computer aided sperm analysis system casa - by Bioz Stars, 2026-05
    86/100 stars

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    computer aided sperm analysis system casa  (Hamilton Thorne Ltd)
    86
    Hamilton Thorne Ltd computer aided sperm analysis system casa
    Computer Aided Sperm Analysis System Casa, supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/computer aided sperm analysis system casa/product/Hamilton Thorne Ltd
    Average 86 stars, based on 1 article reviews
    computer aided sperm analysis system casa - by Bioz Stars, 2026-05
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    computer aided sperm analysis casa  (Hamilton Thorne Ltd)
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    Hamilton Thorne Ltd computer aided sperm analysis casa
    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a <t>CASA</t> system to identify the percentage of <t>motile</t> <t>spermatozoa</t> ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.
    Computer Aided Sperm Analysis Casa, supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/computer aided sperm analysis casa/product/Hamilton Thorne Ltd
    Average 86 stars, based on 1 article reviews
    computer aided sperm analysis casa - by Bioz Stars, 2026-05
    86/100 stars
    		  Buy from Supplier
    computer aided sperm analysis casa system  (Hamilton Thorne Ltd)
    86
    Hamilton Thorne Ltd computer aided sperm analysis casa system
    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a <t>CASA</t> system to identify the percentage of <t>motile</t> <t>spermatozoa</t> ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.
    Computer Aided Sperm Analysis Casa System, supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/computer aided sperm analysis casa system/product/Hamilton Thorne Ltd
    Average 86 stars, based on 1 article reviews
    computer aided sperm analysis casa system - by Bioz Stars, 2026-05
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    casa (computer-aided sperm analysis)  (Hamilton Thorne Ltd)
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    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a <t>CASA</t> system to identify the percentage of <t>motile</t> <t>spermatozoa</t> ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.
    Casa (Computer Aided Sperm Analysis), supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    computer-aided sperm analysis (casa) program ht casaceros ii  (Hamilton Thorne Ltd)
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    Hamilton Thorne Ltd computer-aided sperm analysis (casa) program ht casaceros ii
    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a <t>CASA</t> system to identify the percentage of <t>motile</t> <t>spermatozoa</t> ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.
    Computer Aided Sperm Analysis (Casa) Program Ht Casaceros Ii, supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sperm class analyzer (sca®) computer-aided sperm analysis (casa) system  (Microptic SL)
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    Microptic SL sperm class analyzer (sca®) computer-aided sperm analysis (casa) system
    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a <t>CASA</t> system to identify the percentage of <t>motile</t> <t>spermatozoa</t> ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.
    Sperm Class Analyzer (Sca®) Computer Aided Sperm Analysis (Casa) System, supplied by Microptic SL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    computer-aided sperm analysis (casa; sca® system)  (Hamilton Thorne Ltd)
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    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a <t>CASA</t> system to identify the percentage of <t>motile</t> <t>spermatozoa</t> ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.
    Computer Aided Sperm Analysis (Casa; Sca® System), supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/computer-aided sperm analysis (casa; sca® system)/product/Hamilton Thorne Ltd
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    computer-aided sperm analysis casa  (Hamilton Thorne Ltd)
    90
    Hamilton Thorne Ltd computer-aided sperm analysis casa
    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a <t>CASA</t> system to identify the percentage of <t>motile</t> <t>spermatozoa</t> ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.
    Computer Aided Sperm Analysis Casa, supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/computer-aided sperm analysis casa/product/Hamilton Thorne Ltd
    Average 90 stars, based on 1 article reviews
    computer-aided sperm analysis casa - by Bioz Stars, 2026-05
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    computer-aided sperm analysis system casa  (Hamilton Thorne Ltd)
    90
    Hamilton Thorne Ltd computer-aided sperm analysis system casa
    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a <t>CASA</t> system to identify the percentage of <t>motile</t> <t>spermatozoa</t> ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.
    Computer Aided Sperm Analysis System Casa, supplied by Hamilton Thorne Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/computer-aided sperm analysis system casa/product/Hamilton Thorne Ltd
    Average 90 stars, based on 1 article reviews
    computer-aided sperm analysis system casa - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a CASA system to identify the percentage of motile spermatozoa ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.

    Journal: eLife

    Article Title: Sperm motility in mice with oligo-astheno-teratozoospermia restored by in vivo injection and electroporation of naked mRNA

    doi: 10.7554/eLife.94514

    Figure Lengend Snippet: ( A ) Adult Armc2 KO mouse testes were injected with a solution containing Armc2 -mRNA. After injection, the testes were electroporated. At different times (3-, 6-, 10-, 15-, 21-, 28-, and 35-day post -injection), sperm were extracted from the cauda epididymis of the injected testis, and the sample was then examined with a CASA system to identify the percentage of motile spermatozoa ( A1 ). n = 2 for day 15, n = 3 for days 3, 6, and 21, n = 4 for day 10, and n = 5 for days 28 and 35. ( A2 ) Sperm motility parameters of Armc2 −/− -rescued sperm in comparison to Armc2 +/+ sperm. The motility parameters measured were: averaged path velocity (VAP); straight line velocity (VSL); curvilinear velocity (VCL); amplitude of lateral head displacement (ALH); beat cross frequency (BCF); straightness (STR); linearity (LIN). Black dots: sperm cells from Armc2 null mice, green dots: sperm cells from Armc2 null mice 35 days after injection with Armc2 -mRNA. Results are expressed as mean ± SD. ( A3 ) Sperm motility population of Armc2 −/− -rescued sperm in comparison to Armc2 −/− sperm. Black column: sperm cells from Armc2 null mice, green column: sperm cells from Armc2 null mice 35 days after injection with ARmc2 -mRNA. Statistical significance was verified using a Mann–Whitney sum test. Data are displayed as mean ± SEM. p values of *≤0.05, **≤0.01, or ***≤0.001 were considered to represent statistically significant differences. ( B ) Morphology of sperm cells in Armc2 KO mice injected or not with Armc2 -mRNA. ( B1, B2 ) Microscopic observation of epididymal sperm cells from a mature WT mouse. ( B3, B4 ) Epididymal sperm cells from a mature Armc2 KO mouse 35 days after injection/electroporation with Armc2 -mRNA. ( B5, B6 ) Epididymal sperm cells from a control Armc2 KO male. Normal sperm cells were observed in the injected condition with Armc2 -mRNA (white arrows). Scale bars: 10 µm.

    Article Snippet: Motility of the spermatozoa was evaluated at 37°C with an Olympus microscope and Computer Aided Sperm Analysis (CASA) (CEROS II apparatus; Hamilton Thorne, Beverley, MA, USA).

    Techniques: Injection, Comparison, MANN-WHITNEY, Electroporation, Control